Richter-Helm BioLogics purifies recombinant proteins of various origins, from E.coli and yeast. Cell disruption, renaturisation and chromatographic purification processes are developed, optimised and scaled up to industrial level.
Numerous therapeutically effective proteins are stored in the form of insoluble inclusion bodies in expression systems of procaryotic origin. One of Richter-Helm BioLogics core competences is the in-vitro renaturisation of proteins from inclusion bodies on an industrial scale.
We also boast of expert know-how in the field of processing proteins which are secreted in (semi-)active form into the surrounding medium.
Apart from our competence in the production of proteins, we can also look back on more than 10 years' experience in the processing of plasmid DNA.
We focus in particular on the purification of proteins and plasmid DNA, because the purity of the active components we manufacture has a crucial influence on the effectiveness and safety of the final pharmaceutical product.
Richter-Helm BioLogics employs experienced staff and the latest equipment to develop and optimise cost-efficient processes within a reasonable period of time.
We choose the purification process according to the properties of the product in order to remove all traces of undesirable impurities such as host cell protein, DNA and endotoxins. For this purpose, we use several different methods including precipitation techniques, filtration, ion exchange, affinity, hydrophobic interaction and size-exclusion chromatography.
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